Sterile Nylon Syringe Filters 0.22 µm 13mm | Greyhound Chromatography Laboratory Supplies

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Sterile Nylon Syringe Filters 0.22 µm 13mm

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Why filter your samples for HPLC?

The main source of particle contamination in HPLC columns originate in the sample to be analysed. Therefore, the final preparation step prior to sample injection into the HPLC instrument is to remove any small particulates from the sample by filtration. Removal of the solid materials is very important as they can interfere with the compound of interest and easily clog up the column being used. This will inevitably have a detrimental effect on the performance of the column, i.e. back pressure, peak size, retention time, peak shape. Severe contamination can lead to the column being irreversibly blocked and therefore having to be replaced. Other costs can include instrument downtime, and the loss of valuable samples.

Sample Types

Samples differ in a variety of ways, they may be heavily loaded with fine or coarse particulates, dissolved in aqueous or organic solvents with varying viscosities. To facilitate the most appropriate sample preparation result, filters should be optimised to match the particular requirements of each kind of sample being injected.

Standard Samples
A matrix of 5-10mL is the most common sample volume, with low viscosity, low particle contamination and dissolved in either an organic or an aqueous solvent. Greyhound Q-Fil Regenerated Cellulose Syringe Filters are an excellent choice for such samples. Their inert housing manufactured from high density medical grade virgin polypropylene, complete with the integrated Regenerated Cellulose (RC) membrane has a high chemical resistance against the most common HPLC solvents and is very suitable for aqueous samples. Every batch is HPLC-tested for low extractables. Whilst 0.45µm is the most commonly used membrane porosity, the 0.22µm membrane provides improved purity when using capillary columns or HPLC packings with a particle size of 3µm or less.

Demanding Samples
More difficult samples which contain high loads of particulates, high viscosity or unusually high volumes place additional challenges on the user when preparing samples for HPLC analysis. The particles have a tendency to block the filter, high viscosities will decrease the flow rate and high volumes increase the time required to complete the filtration process. These challenges often result in the user applying a greater degree of manual pressure to the process and potentially exceeding the maximum pressure limit of the filter with a subsequent risk of bursting the housing. As a result, the sample will be lost and a potential safety risk can arise if corrosive solvents or harmful chemicals are being filtered. To assist in overcoming these challenges, the use of Q-Fil Syringe Filters with a built in Glass Microfiber pre-filter are recommended. The pre-filter removes the larger particulates from the sample leaving only the smaller particulates to be filtered by the membrane. These filters increase the flow rate through the unit resulting in a higher volume of sample to be filtered and less pressure being required by the user.

Small Volume Samples
Smaller volume samples are often valuable and require special attention. Any loss of the analyte due to adsorption by the filter membrane or housing or a large dead volume can have a detrimental effect on the analysis. Smaller filters of 13mm and 4mm diameter decrease the dead volume to less than 25uL and 8uL respectively making them ideal for smaller volume samples.

Sample filtration is an important factor in ensuring that HPLC analysis is achieved in an efficient and cost effective way. The extensive range of Q-Fil Syringe Filters have been developed to meet the demands of the analyst and the different sample matrices being used and without losing any of the important sample.

For over 35 years Greyhound Chromatography has been supplying high quality Chromatography consumables to laboratories around the world. Greyhound’s extensive range covers all areas of Environmental, Petrochemical, Food, Forensics, Chemical and Pharmaceutical analysis. Backed by a highly experienced technical services team, Greyhound is the preferred source amongst today’s analysts.

Greyhound Chromatography is pleased to supply Greyhound's Q-Fil Syringe Filters to Research and Analysis laboratories worldwide.

Price shown is correct 24 April 2018. Please visit our webshop for latest prices. Please send email to for quotations for multiple purchases.


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