Purospher STAR NH2, 5µm, 120Å, 250mm x 4.6mmID, Hibar RT HPLC ColumnPurospher™ STAR NH2 (Amino) columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water.
In terms of their polarity, these columns lie between bare silica (normalphase chromatography) and reversed-phase silica (reversed-phase chromatography). Hence, Purospher™ STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3+X-) and therefore display the characteristics of a weak anion exchanger.
Medium polarity Purospher™ STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed phase and normal-phase conditions. However, retention
is weaker than on silica or RP-supports.
