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Water Determination In Nitrogen Bases | Honeywell | Greyhound Chromatography

Water determination in nitrogen bases by Honeywell

HYDRANAL™ Laboratory Report L 288

Nitrogen Bases by Honeywell

Compounds containing nitrogen are commonly occurring and are very important in organic chemistry. Many active pharmaceutical ingredients possess nitrogen in their chemical structure. Different types of amines are often ingredients of drugs.

Procedures listed in the existing literature about water determination in nitrogen bases are rather vague. Therefore we tried to make more precise statements and develop more exact titration methods.

We examined over 100 separate amines using the 3 common titration techniques:

  • volumetric titration with one-component reagents
  • volumetric titration with two-component reagents
  • coulometric determination

We followed our special procedure for each technique and measured: time for titration, end-point stability, and recovery rate of added water (in the presence of the sample being investigated).

Tested titration media were acidified by additions of benzoic acid, salicylic acid or sulfite buffer. In some cases ready to use solution, Hydranal-Buffer Base, was used.

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